Effect of Hydroxyapatite Nanoparticles of Different Concentrations on Rat Osteoblast
Nano-HAP (10-20nm) were obtained from East China University of Science and Technology. The osteoblasts were primary cultured from rat calvaria and then treated with five different concentrations(20，40，60，80，100μg/ml) of nano-HAP, the osteoblasts without nano-HAP was used as control group. Inhibition ratio, apoptotic rate were evaluated by MTT assay and flow cytometry (FCM), respectively. The specific surface area of nano-HAP was detected by BET. All date were expressed as mean ± standard deviation.Statistical analysis was performed by t test using software SPSS11.0 for Windows. The results indicated that the nano-HAP could inhibit the growth of osteoblasts in a dose-dependent manner. When the concentrations of nano-HAP were 20, 40, 60, 80, 100μg/ml, the inhibition ratio were 2.8％, 22.2％, 26.9％, 38％ and 47.7％, and the apoptotic rate were 4.63％, 6.75％, 9.47％, 11.49％, 17.22％, respectively, which were obviously higher than that of control group. The nano-HAP significantly induced apoptosis in osteoblasts. There were the same tendency that the apoptotic and inhibition ratio of osteoblasts were rising with the increasing of the concentration of the nano-HAP. The specific surface area of nano-HAP was 148.140m2/g.
Hydroxyapatite Nanoparticle Osteoblasts Inhibiton Apoptosis
Zhengli XU Jiao SUN Changsheng Liu Jie Wei
Ninth Peoples Hospital, School of Medicine, Shanghai Jiaotong University Shanghai Biomaterials Rese Key Laboratory for Ultrafine Materials of Ministry of Education Engineering Research Center for Biom
国际会议
重庆
英文
1364-1369
2008-06-09（万方平台首次上网日期，不代表论文的发表时间）