Determination of Plasma Free Fatty Acids in Diabetic Rats by HPLC with Electrochemical Detection and the Relation between FFAs and Valproic Acid
The presence of free fatty acid (FFA) in unbuffered solution containing 2-methyl-l,4-naphtoquinone (VK3) causes a prepeak of the quinone reduction wave on the voltammogram, and the prepeak height is increased with the FFA concentration. Based on this catalytic prepeak, we previously developed a new HPLC with electrochemical detection (HPLC-ECD) using VK3 for determining plasma FFAs. In the previous paper 1, we reported the monitoring plasma FFA with blood glucose level before and after meal ingestion. It has been interesting to clearify the relation between FFAs and valproic acid (VA), a drug for seizures and acute psychotic mania. In this study, the HPLC-ECD system has been improved in terms of sensitivity and separation of each FFAs and of VA by a C30 microbore column and 3,5-di-t-butyl-l,2-benzoquinone (DBBQ) as the quinone reagent, and the determination of plasma FFAs and VA in normal and diabetic rats was carried out using the present method.
Fumiyo KUSU Takafumi OHTSUKA Nana ISHII Akira KOTANI
School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1, Horinouchi, Hachioji, Tokyo 192-0392, Japan
国际会议
The Twelfth International Symposium on Electroanalytical Chemistry(第十二届国际电分析化学会议 12th ISEC)
长春
英文
33-34
2009-08-12（万方平台首次上网日期，不代表论文的发表时间）