Electrochemiluminescence Immunosensor for Human IgG with Ru(bpy)32+-Encapsulated Liposomes as Labels
Since it was first discovered and reported by Bangham group in 1965 1, liposomes have gained considerable attention as a versatile carrier for a wide spectrum of species and have been used in a variety of applications such as gene therapy, drug delivery cosmetic skin conditioners, and immunodiagnostics 2. Unlike metal particles and latex beads, of which only the outer surface can function in the detection process, the interiors of liposomes can be engineered to encapsulate a great amount of reporter molecules, such as fluorescent dyes, electroactive markers, and enzymes, therefore, could provide signal amplification 3. During the past several years, electrochemiluminescence (ECL) has attracted great interest as an important and valuable detection method in analytical chemistry. Tris(2,2-bipyridyl)ruthenium(II) (Ru(bpy)32+) and its derivatives are the most widely used complexes for analytical investigations and applications such as DNA analysis and immunoassay because of their excellent intrinsic characteristics and abilities to produce ECL with high efficiency 4.
Ling LI Dongyan SUN Haivan WANG
Anhui Key Laboratory of Chemo-biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, P. R. China
国际会议
The Twelfth International Symposium on Electroanalytical Chemistry(第十二届国际电分析化学会议 12th ISEC)
长春
英文
125-126
2009-08-12（万方平台首次上网日期，不代表论文的发表时间）