DNA Functionalized EQCM Biosensor for the Detection of Bacillus Anthracis
The rapid detection of Bacillus anthracis has gained great attention since the anthrax spore bioterrorism attacks in America in 2001 1, 2. In this work, a DNA probe functionalized quartz crystal microbalance (QCM) biosensor was developed to detect Bacillus anthracis based on the recognition of its specific DNA fragment in chromosomal and plasmid PXO1.First, primers were designed towards the highly specific DNA sequences, i.e., 168 bp Ba813 gene in chromosomal and 340 bp fragment of pag gene in pXO1.Then, upward primer, also as probe, was decorated with mercapto-group at its 5 end, and then immobilized onto QCM Au surface through self-assembling between Au-S bond, for the hybridization with target ss-DNA sequence, which was obtained in the method of asymmetry PCR or thermal denature after normal PCR.
Rongzhang HAO Xian-En ZHANG Guomin ZUO Hongping WEI Ruifu YANG Zhiping ZHANG Zhenxing CHENG DianbingWANG Zongqiang CUI
The No. 3 Department, Institute of Chemical Defence, P.O. Box 1048, Beijing 102205, China State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 43 State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academ
国际会议
The Twelfth International Symposium on Electroanalytical Chemistry(第十二届国际电分析化学会议 12th ISEC)
长春
英文
157-158
2009-08-12（万方平台首次上网日期，不代表论文的发表时间）