Fabrication of an Intracellular Enzyme Biosensor by Sacrificed Cell Cultivability with Eztracellular Scaffold
The living cells or the purified enzymes generally have been applied for industrial bioprocesses and biosensors. However, they preparation methods have some disadvantages, such as harsh conditions for cell growth, the substrate and product control for the living cells (1), complicated operation, and expensive cost (2). The cells, in the state which were between the programmed survival forms and the deaths door, were called viable but nonculturable (VBNC) cells (3). Under the VBNC state, bacteria are unable to form colonies in normal growth media but are still viable and endowed with metabolic activity.
LING LIU Li SHANG Dan LI Shaojun DONG
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
国际会议
The Twelfth International Symposium on Electroanalytical Chemistry(第十二届国际电分析化学会议 12th ISEC)
长春
英文
168-169
2009-08-12（万方平台首次上网日期，不代表论文的发表时间）