Coupling of Fluorescence Correlation Spectroscopy with Stage-Scanning Confocal Fluorescence Imaging System and its Applications in Single Cells Analysis
Single-cell analysis including assay of cell components and study on biochemical processes in living cells becomes extremely important in biology and biomedicine. So far, there are mainly two types of single-cell analysis methods based on micro-nano separation (e.g.: capillary electrophoresis) and fluorescence microscopy (e.g.: confocal laser scanning microscopy). In situ single-cell analysis based on fluorescence microscopy allows us to observe the distribution of biomolecules and investigate molecular movement in living cell, interaction between different molecules, conformational changes under different environments. At present, commonly used methods based on fluorescence microscopy mainly include fluorescence recovery after photobleaching (FRAP), fluorescence resonance energy transfer (FRET), fluorescence correlation spectroscopy (FCS) 1, multiphoton-excited fluorescence microscopy (MPEFM), fluorescence lifetime imaging (FLIM), total internal reflection fluorescence microscopy (TIRFM), and so on.
Zhancheng XU Xin ZHU Bocheng ZHANG Chaoqing DONG Jicun REN
School of Chemistry & Chemical Engineering, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai, 200240, P. R. China
国际会议
The Twelfth International Symposium on Electroanalytical Chemistry(第十二届国际电分析化学会议 12th ISEC)
长春
英文
194-195
2009-08-12（万方平台首次上网日期，不代表论文的发表时间）