Bifunctional Magnetic SERS Probes based on Thrombin Aptamer and its Application in Thrombin Detection
Aptamer has been widely used in specific molecular recognitions from inorganic and organic species to proteins and cells 1. Until now, many methods based on aptamer to recognize specific targets have been developed such as surface plasmon resonance (SPR) 2, atomic force microscopy (AFM) 3, fluorescence 4, colorimetry 5, electrochemistry 6, and so on. Surface enhanced Raman scattering (SERS), which is a powerful tool in biologic analysis for its high sensitivity and little disturbance from water, is also used to fabricate new sensors based on aptamer to detect protein with quite low detection limit and high selectivity 7. However, for the existed aptasensors, nonspecific absorption is a problem which would influence the sensitivity and reliability. To reduce this influence, complex processes, such as repeated wash or blocking with special molecule, are usually needed. Herein we developed bifunctional magnetic SERS probes based on aptamer which could simplify the process and reduce the influence of nonspecific absorption. Meanwhile the detection limit is comparable to other methods.
Wen REN Yuling WANG Tiantian WANG Erkang WANG
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
国际会议
The Twelfth International Symposium on Electroanalytical Chemistry(第十二届国际电分析化学会议 12th ISEC)
长春
英文
258-259
2009-08-12（万方平台首次上网日期，不代表论文的发表时间）