MICROPLATE ENZYME ASSAY FOR SCREENING LIPOXYGENASES TO DEGRADE WOOD EXTRACTIVES
Wood extractives are known to cause a range of problems in pulp and paper manufacturing. Soybean lipoxygenase enzyme treatments to degrade wood extractives present in thermomechanical pulp (TMP)samples resulted in a 15% reduction in total extractives. However, commercially available lipoxygenase is currently not cost effective for this application. A 96-well UV microplate assay was developed to screen enzymes from fungal species for a more cost-effective alternative lipoxygenase. The substrate used for this assay is linoleic acid, a predominant fatty acid in wood extractives. The enzyme activity and reaction kinetics determined by this microplate assay were compared to those obtained from a conventional bench scale assay. A number of hydrolytic enzymes, and other types of oxidases were also tested by this protocol to examine the specificity of the assay. The results from this study show that the developed microplate assay can provide an inexpensive method for accelerated screening of a large number of enzymes to identify potential oxidative enzymes with specific action in degrading wood extractives.
lipozygenase eztractives degradation
David Nguyen Xiao Zhang Mike G Pake Adrian Tsang Sylvie Renaud
Pulp and Paper Research Institute of Canada (PAPRICAN),570 boul. St-Jean, Pointe-Claire, Quebec, H9R Pulp and Paper Research Institute of Canada (PAPRICAN),570 boul. St-Jean, Pointe-Claire, Quebec, H9R Centre for Structural and Functional Genomics, Concordia University,7141 Sherbrooke Street West, Mon
国际会议
广州
英文
72-77
2006-11-08（万方平台首次上网日期，不代表论文的发表时间）