EFFECTS OF A LOW-MOLECULAR-WEIGHT PC REDUCER FROM PHANEROCHAETE CHRYSOSPORIUM ON LIGNIN BIODEGRADATION
Biodegradation of lignin by ligninolytic fungi and their enzymes is an important process in biopulping or biobleaching. A novel low-molecular-weight compound with reductive activity, named Pc reducer, was purified from the liquid culture of a white-rot basidiomycete fungus Phanerochaete chrysosporium. Its molecular weight was 1017 Da estimated by ESI-MS and MALDI-TOF. According to MS and NMR, Pc reducer was likely to be a polyamide compound composed of the same unit of 113 Da. Pc reducer had high thermostability and was oxygenated slowly by O2.It could chelate Fe3+ and reduce it to Fe2+. Pc reducer could reduce hydroxyl radical HO and 4-maleimideTEMPO under certain conditions. The concentrations of Pc reducer threw complicated influences on the activities of lignin peroxidase and Mn-dependant peroxidase. Moreover, it could inhibit the repolymerization of products from oxidation of phenolic lignin-model compounds by peroxidases. These characters provided Pc reducer the potential ability to improve the lignin biodegradation efficiency of ligninolytics enzymes in biopulping or biobleaching.
Hu Ming Zhang Weican Lu Xuemei Gao Peiji
State Key Laboratory of Microbiol Technology, Shandong University, Jinan, 250100, China
国际会议
广州
英文
376-381
2006-11-08（万方平台首次上网日期，不代表论文的发表时间）