High-Contrast Multimodel Nonlinear Optical Imaging of Collagen and Elastin
Collagen and elastin, as the major components in the extracellular matrix (ECM), are intrinsic indicators of physiological and pathological states. Here, we have developed a high-contrast multimodel nonlinear optical imaging technique to imaging collagen and elastin by detecting simultaneously two photonexcited fluorescence (TPEF) from elastin and secondharmonic generation (SHG) from collagen. Our results show that this technique can obtain a high-contrast TPEF/SHG image in human dermis and permit direct visualization of collagen and elastin. It was shown that the technique can provide collagen and elastin structural information to determine collagen and elastin fibril orientation and distribution and acquire some morphometric properties. It was found that the in-depth TPEF/SHG imaging and 3-d reconstruction of TPEF/SHG images can extract more collagen and elastin structural and biochemical information. The study results suggest that the highcontrast multimodel nonlinear imaging provides a powerful tool to study ECM intrinsic components and has the potential to provide more important information for the diagnosis of tissue.
S M Zhuo J X Chen T S Luo H L Chen J J Zhao
Key Laboratory of Optoelectronic Science and Technology for Medicine (Fujian Normal University), Min Department of Skin, Affiliated Xiehe Hospital, Fujian Medical University, Fuzhou 350001, China
国际会议
第四届仪器科学与技术国际会议( 4th International Symposium on Instrumentation and Science and Tcchnology)
哈尔滨
英文
1476-1481
2006-08-08（万方平台首次上网日期，不代表论文的发表时间）