Development of a DNA Microarray for Detection of Wastewater Bacterial Pathogens

摘要：

1.Introduction Despite rapid advancements in water quality management technologies,waterbome pathogens still remain as one of the major environmental threats to human health. The rapid,specific,sensitive detection of pathogenic baeteria is an important challenge in modern water microbial quality monitoring. The microarray assay have recently emerged as a promising alternative for environmental pathogen monitoring.Because of its high throughput design,microarray allows for processing of multiple samples and monitoring up to tens of thousands of genes in a single assay. The main objective of this study was to design and validate an oligonucleotide microarray to detect 6 bacterial pathogens.Moreover,the suitability of propidium monoazide sample treatment in combination with microarray detection was validated.

作者: Shu-Xu WU Miao HE

作者单位: State Key Joint Laboratory on Environment Simulation and Pollution Control,Department of Environmental Science and Engineering,Tsinghua University,Beijing China,100084

会议类型: 国际会议

会议名称: International Conference on Environment Simulation and Pollution Control(第六届环境模拟与污染控制学术研讨会)

会议地点: 北京

会议语种:英文

页码: 164-165

在线出版日期: 2009-11-01（万方平台首次上网日期，不代表论文的发表时间）