Development of novel evanescent wave biosensor for detection of DNA

摘要：

@Evanescent wave fiber optic biosensors have been developed to determine various trace amounts of targets interest based on the principle of total internal reflect fluorescent (TIRF)1-3, which boast promising advantages such as feasible miniaturization, sensitivity, cost-effectiveness, and rapid measurements. Previously, we developed a prototype set-up of evanescent wave all-fiber biosensor, in which both the transmission of the excitation light and the collection and transmission of the fluorescence are achieved by fiber optics through the single-multi fiber optic coupler. Here we used this all-fiber bioassay read-out system to develop a novel DNA biosensor, which could detect DNA with a linear calibration curve observed in the 1 nM to 10 μM range.

作者: Feng Long Hanchang Shi Shuxu Wu Tiezheng Tong Miao He Shuang Han

作者单位: Environmental Simulate and Pollution Control State Key Joint Laboratory,Dept.of Environment Science and Engineering,Tsinghua University,Beijing,100084,China

会议类型: 国际会议

会议名称: International Conference on Environment Simulation and Pollution Control(第六届环境模拟与污染控制学术研讨会)

会议地点: 北京

会议语种:英文

页码: 166-167

在线出版日期: 2009-11-01（万方平台首次上网日期，不代表论文的发表时间）