Amperometric Immunosensor for α -Fetoprotein Antigen in Human serum Based on Co-immobilizing Dinuclear Copper Complez and Gold Nanoparticle Doped Chitosan Film
A sensitive amperometric immunosensor for α -fetoprotein (AFP), a tumor marker for the diagnosis of hepatocellular carcinoma (HCC), was constructed , The immunosensor is prepared by co-immobilizing Cu2(phen)2Cl2 (μ-Cl)2 (CuL), nano-Au/Chitosan(Chit) composite, horseradish peroxidase (HRP) and AFP antibody(anti-AFP) on a glassy carbon electrode (GCE). Firstly ,CuL was irreversibly absorb on GCE electrode through π-π stacking interaction; then nano-Au/Chit composite was immobilized onto the electrode because of its excellent membrane-forming ability, finally HRP and anti-AFP was adsorbed onto the surface of the gold nanoparticles to construct GCE | CuL/nanoAu-chit /HRP/anti-AFP immunosensor. The preparation procedure of the electrode was characterized by electrochemical and spectroscopy method. The results showed that this immunosensor exhibited an excellent electrocatalytic response to the reduction of hydrogen peroxide (H2O2) without the aid of an electron mediator, offers a high-sensitivity (1710 nA·Ng-1·Ml-1) for the detection of AFP and has good correlation for detection of AFP in the range of 0.2 to 120.0 ng/ml with a detection limit of 0.05 ng/ml. The biosensor showed high selectivity as well as good stability and reproductivity.
Ning Gan Ling Hua Meng Feng Wang
The State Key Laboratory Base of Novel Functional Materials and Preparation science,Faculty of Material Science and Chemical Engineering of Ninbo University,Ningbo,315211,China
国际会议
The 8th China International Nanoscience and Technology Symposium(2009第八届中国国际纳米科技湘潭研讨会Cinsts09)
湖南湘潭
英文
1-6
2009-10-23（万方平台首次上网日期，不代表论文的发表时间）