Determination of Hemoglobin Ozygen Saturation and Total Hemolgobin Concentration from Turbid Media Using Absorption Spectroscopy
Oxygen is a versatile molecule involved in a wide range of biological processes. Under physiological conditions, oxygen reacts with hemoglobin to form oxyhemoglobin (OxyHb) at a very high rate. The degree of their combinations is described as blood oxygen saturation (SO2) that has been estimated as well as hemoglobin concentration mostly by Near Infrared Spectroscopy (NIRS) by now. However, the size of the probe used in this technology was too large to apply to the inner body, such as deep brain tissue. The aim of this work was to evaluate the possibility of real-time estimating these parameters from rat cortex by in vivo absorption spectroscopy using a needle-like probe. An artificial neural network (ANN) trained by in vitro data was used. The validity of this approach was studied by decreasing the endogenous OxyHb level with asphyxia. Data indicate that in vivo visible spectroscopy in combination with the ANN fitting method may be a useful tool for gaining real-time insight into SO2 and hemoglobin concentration even from deep brain tissue or small cerebral areas.
ozyhemoglobin blood ozygen saturation hemoglobin concentration in vivo Absorption spectroscopy artificial neural network
DAI Li-Juan HUA Guo-Ran QIAN Zhi-Yu
Colledge of Mechanics, Nantong University, 226019 Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, 210016
国际会议
南京
英文
346-353
2009-10-23（万方平台首次上网日期，不代表论文的发表时间）