Adsorption and Inhibition of Butyrylcholinesterase by Different Nanoparticles
Nanoparticles may have potential neurotoxicity. This study used 5 nanoparticles including 2 metal nanoparticles and 3 oxide nanoparticles to test their adsorption and inhibition on BChE. We used a modified Ellman method to measure BChE activity. At 800 mg·L-1, the adsorption and inhibition of BChE by Cu were the highest, 54% and 86%, respectively. While Al nanoparticles showed the lowest adsorption (6.8%) and inhibition rates (3.3%). Inhibition was caused mainly by the adsorption of BChE on tested nanoparticles and partly by ions released in nanoparticle suspensions. Inhibition of BChE by Cu2+ ions was higher than 39%. Other ions had slight or little effect on BChE activity. The contribution of ions to nanoparticle inhibition followed a decreasing sequence of Al (66%)>Cu (46%)>Al2O3 (44%)>SiO2 (4%). Our results indicate that these nanoparticles may have neurotoxicity.
Copper Enzyme Neurotozicity Metal ion Biomarker
Zhenyu Wang Kai Zhang Jian Zhao Fengmin Li Dongmei Gao Baoshan Xing
College of Environmental Science and Engineering,Ocean University of China,Qingdao 266100,China Department of Plant,Soil and Insect Sciences,University of Massachusetts,Amherst MA 01003,USA
国际会议
杭州
英文
262-264
2009-10-10（万方平台首次上网日期，不代表论文的发表时间）