Effect of crylAb Gene Transformation on the Microbial Mediated Decomposition of Rice Residues under Intensive Rice Cropping System
Although genetically modified (GM) plants can offer many benefits, the planting of transgenic crops has raised a number of concerns, including the ecological impact of these plant residues on soil ecosystems. In this study, the effects of rice expressing the Bacillus thuringiensis Cry1Ab protein (Bt rice) on the residue decomposition processes were assessed in comparison with parental rice variety (non-Bt rice) under rapeseed-rice cropping system and surface or incorporated placement conditions. Bacterial and fungal communities associated with residue decomposition were studied by terminal restriction fragment length polymorphism (T-RFLP) method and additive main effects multiplicative interaction (AMMI) analysis model. After 276 days and residue decomposition in the field condition, bacterial and fungal communities associated with decomposition were strongly affected by the temporal factor which represented the grouping of T-RFLP fingerprint according to the time factor alone IPC 1. The placement effect on soil bacterial and fungal communities was also detected which represented the grouping of T-RFLP fingerprint alone iPC 2. Although some differences were found between Bt and non-Bt rice varieties in some special stages and placements, the impact of cry1Ab gene transformation on microbial mediated decomposition was lower than temporal and placement factors.
Bt transgenic rice Microbial community T-RFLP AMMI
Haohao Lu Weixiang Wu Yingxu Chen
College of Environmental and Natural Resource Sciences,Zhejiang University,Hangzhou 310029,China Col College of Environmental and Natural Resource Sciences,Zhejiang University,Hangzhou 310029,China
国际会议
杭州
英文
325-327
2009-10-10（万方平台首次上网日期，不代表论文的发表时间）