Effects of Hydrogen Perozide on 15-kDa Selenoprotein Transcription and Translation
(0) Selenoproteins are proteins containing selenocysteine,in which the sulfur was replaced with selenium (Se).Seleno-cysteine is the 21~(st) amino acid coded by the common stop codon UGA Translation of selenoprotein is combined with (SEC1S) located at 3UTR and some translation factors specific for selenoproteins. 15-kDa selenoprotein (Sepl5) is a protein containing seleno-cysteine,whose molecular weight of mature protein is about 15 kDa (Gladyshev et al.,1998).It is reported that Sepl5 (Hatfield et al.,2006) and Se are respectively related to cancer prevention (Kumaraswamy,2000),while selenoprotiens are involved with antioxidant activity (Huang and Xu,2008).Stable cells transected with Sep 15 can increase cell vitality compare to control after treatment with hydrogen peroxide (H_2O_2) (Wu et al.,2003). to elucidate whether Sep 15 expression relates to the oxygen stress resistance of the cells,a human normal hepatocyte cell line-HLO2 was treated with H_2O_ 2. The mRNA levels of Sep 15 were quantified by real time PCR and the protein level of Sep 15 was detected by western blot.In order to select suitable concentrations of H_2O_2 for treatment,cell vitality was detected 72 hrs after treatment.
Jing Tian Qiong Liu Jia-Zuan Ni
Shen Zhen Uinversity,Shen Zhen 518060.China Changchun Institute of Applied Chemistry,Chinese Academy Shen Zhen Uinversity,Shen Zhen 518060.China
国际会议
The First International Conference on Selenium in the Environment and Human Health(第一届硒与环境和人体健康国际会议)
苏州
英文
63-64
2009-10-01（万方平台首次上网日期，不代表论文的发表时间）