Design of Cell-recognition Biomaterials for Regenerative-medicine and Cell-Targeting Medicine
Treatment of a physiological disorder in the genetic level (gene therapy) and induction of a specific immunity by means of a genetic material (genetic vaccination), are considered two revolutionary approaches for clinical medicine. The implementation strategies for these basic concepts demand a vehicle for nucleic acid delivery. Viral delivery systems, although highly efficient, possess severe limitations in terms of life safety and thus non-viral synthetic systems have become increasingly desirable. Intensive efforts for the last 3 decades enabled the development of a lot of synthetic devices, most of which belong to cationic lipids, peptides and other polymers, but comparatively little attention was paid to inorganic materials (1). We have recently reported on the development of the simplest, but highly efficient gene delivery device based on generated carbonate apatite nano-crystals having high affinity to DNA but fast dissolution kinetics for effective release of DNA during vesicular acidification and thus resulting in 5 to 100-fold higher transgene expression than the existing ones (2). Additionally, flexibility in modulating crystal dissolution kinetics enabled to control intracellular DNA release and an intermediate rate of DNA release significantly enhanced survival of DNA and thus subsequent protein expression to a notable extent.
Toshihiro Akaike
Frontier Research Center & Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology,4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan
国际会议
International Symposium on Crystal Engineering and Drug Delivery System 2009(2009晶体工程与药物传送系统国际会议)
天津
英文
315-316
2009-09-05（万方平台首次上网日期，不代表论文的发表时间）