Novel in situ Forming Hydrogels for Cartilage Engineering
In this presentation two approaches for the preparation and evaluation of in situ forming hydrogels for cartilage repair will be discussed. In the first approach enzymatic crosslinking of dextran-tyramine (Dex-TA) conjugates in the presence of horseradish peroxidase and hydrogen peroxide was applied in the preparation of hydrogels. Depending on the molecular weight of the dextran (Mn of 14000 or 31000 g/mol) and the degree of substitution (DS of 5, 10 or 15) with tyramine (TA) groups, the gelation times ranged from 20 s to 1 min. Hydrogels prepared from Dex31k -TA with a DS of 10 had storage moduli up to 60 kPa. Similar values were found when chondrocytes were incorporated into the hydrogels. Chondrocyte-seeded Dex-TA hydrogels were prepared at a molar ratio of H2O2/TA of 0.2 and cultured in a chondrocyte medium. A live-dead assay and a MTT assay revealed that almost all chondrocytes retained their viability after 2 weeks. Cell division was observed in Dex14k-TA DS 10 and agarose hydrogels. SEM analysis showed that the encapsulated chondrocytes were capable of maintaining their round shape. Histology and immunofluorescent staining demonstrated the production of glycosaminoglycans (GAGs) and collagen type II after culturing for 14 and 21 days. Biochemical analysis showed that GAG accumulation increased with time inside Dex-TA hydrogels. Besides, GAG/DNA for Dex-TA hydrogels was higher than that for agarose at day 28.
R. Jin L.S. Moreira Texeira A. Krouwels P.J. Dijkstra C. van Blitterswijk M. Karperien J. Feijen
University of Twente Faculty of Science and Technology MIRA-Biomedical Technology & Technical Medicine Enschede, The Netherlands
国际会议
International Symposium on Crystal Engineering and Drug Delivery System 2009(2009晶体工程与药物传送系统国际会议)
天津
英文
317-318
2009-09-05（万方平台首次上网日期，不代表论文的发表时间）