Controlled Release of pDNA-PTD Complez for Enhancing Transgene Ezpression in Human Adipose-derived Stem Cell
Tissue engineering scaffolds capable of sustained plasmid DNA release can provide enhanced gene transfection efficiency and prolonged secretion of bioactive protein for new tissue formation. High transfection efficiency could be achieved by employing Hph-1 protein transduction domain (PTD) fused to plasmid DNA (pDNA). Gene expression in cells trasfected by Hph-1 was significantly higher in comparison with standard liposomal transfection. To obtain the controlled delivery of pDNA from polymeric scaffolds, emulsion-coating method has been developed. Plasmid DNA-Hph-1 complexes were emulsified with poly(lactide-co-glycolide)(PLGA) polymer solution, and then the emulsion was coated on the poly(L-Lactic acid)(PLLA) fibrous 3-D scaffold surface. Human adipose-derived stem cells (hASCs) were seeded on each scaffold group, PLLA scaffolds coated with pDNA-PTD complexes (group 1), PLLA scaffolds with bolus delivery of pDNA-PTD complexes (group 2), PLLA scaffolds without pDNA (group 3). Transgene expression by sustained DNA delivery system (group 1) revealed significantly higher and longer even after 2 weeks of culture. Furthermore, protein production increased when the trasnfection time was extended, indicating sustained activity of pDNA-Hph-1 complex. Therefore, this sustained DNA delivery system would be not only an effective gene delivery method, but a valuable tool for stem cell based tissue engineering.
Seung-Jin Lee
Department of Pharmacy, Ewha Womans University, Seoul, Republic of Korea，
国际会议
International Symposium on Crystal Engineering and Drug Delivery System 2009(2009晶体工程与药物传送系统国际会议)
天津
英文
319
2009-09-05（万方平台首次上网日期，不代表论文的发表时间）