Self-assembled lipid/DNA Nanoparticles for pMDI Aerosol Delivery of Genes to the Lung

摘要：

Gene therapeutics including DNAs and siRNAs are considered to have great potentials for the prevention and treatment of lung diseases 1-3. But gene delivery to the mucosal epithelial cells lining the airway surfaces had been difficulty. We reported here a novel self-assembled lipid/DNA nanoparticle system that can deliver genes through out the lung and achieve significant gene delivery efficiencies. The nanoparticles had a particular reverse micelle structure and could be packaged into pressurized metered dose inhaler (pMDI) with high capacities. The nanoparticle aerosols were shown to be able to transfect cultured cells as well as mouse lung with board coverage of the mucosal surfaces. DNA vaccination using such a system resulted in significant antibody responses against influenza viral antigens.

作者: Xu, Yuhong

作者单位: School of Life Science and Biotechology, Shanghai Jiao Tong University, China

会议类型: 国际会议

会议名称: International Symposium on Crystal Engineering and Drug Delivery System 2009(2009晶体工程与药物传送系统国际会议)

会议地点: 天津

会议语种:英文

页码: 366

在线出版日期: 2009-09-05（万方平台首次上网日期，不代表论文的发表时间）