Nanotechnology-based Drug Delivery Systems Targeting to Glioma
Some novel nanoparticulate systems were developed for targeting therapy to glioma, based on modification of the special ligands and PEGylation. The chemical compound and gene drugs have been challenged to target glioma in our study. The receptor-mediated and absorptive-mediated transcytosis should contribute in the active target glioma of drugs carried by the modified nanoparticles. And the cationic bovine serum albumin conjugated stealthy nanoparticulate system (CBSA-NP), the Transferrin conjugated polymersomes (Tf-PO) and nanoparticles were investigated in the glioma model mice respectively. CBSA-NP served as delivery carrier, encapsulated siRNA interference pDNA targeting c-Myc oncogene, whose transfect C6 glioma cells in vitro revealed higher transfected efficiency comparing with those of NP-siRNA system.(48.29%>24.77%). We established the brain glioma model of C6 lines in mice, and measured the tumor volume, the expression of glioma specifically representative protein S-100β and tumor cell apoptosis rate in order to evaluate the CBSA-NP-siRNA gene therapy efficacy and the performance in inhibiting cancer cell proliferation. Results showed that, after a week tail vein injection treatment, the tumor volume of CBSA-NP-siRNA group was 9.64±14.97 mm3，significantly smaller than the volume of control group, which was 97.62±20.12 mm3; the amount of S-100β expression of CBSA-NP-siRNA group reduced dramatically; flow cytometry detection for the cancer cell apoptosis rate of the CBSA-NP-siRNA group was 34.28%, much higher than the rate of the physiological saline control group (1.83%) and NP-siRNA group (21.37%). All results revealed that CBSA-NP-siRNA was an effective gene delivery system for brain glioma targeting.
Jiang Chen Jiang Xinguo Pang Zhiqing Xu Feng Li Jianfeng
Department of Pharmaceutics, School of Pharmacy,Fudan University, Shanghai, China Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai, China
国际会议
International Symposium on Crystal Engineering and Drug Delivery System 2009(2009晶体工程与药物传送系统国际会议)
天津
英文
389-390
2009-09-05（万方平台首次上网日期，不代表论文的发表时间）