Development of Artificial Feeder Layer and Application of 3D Scaffold for the Culture and Proliferation of Cells
Extracellular matrix (ECM) is the extracellular part of tissue that not only provides structural support and anchorage of cells but also plays an important role in cell proliferation, differentiation, migration and cell’s behaviors, prevents the startup of immune system from responding with inflammation and scar tissue at same time. Recently, the complexes of biomaterials and cells have excited distinct attention and extensive research has been developed in regenerative medicine field. And the three-dimension culture process plenty of advantages that it can provide similar environment to it in vivo under the condition of high density and continuous culture while the microorganisms pollution can also be prevented. Then it can be applied into the fields of culture of different cells, development of artificial organs and evaluation of pharmacokinetics. In this work poly-L-lactide (PLLA), polyethylene (PE), polyethylene terephthalate (PET) and polystyrene (Pst) fibers were produced by electrospinning method and the mechanical properties were measured as well to evaluate the possibility of the application in regenerative medicine, such as the bioreactor for 3D culture. Later the attachment property of the polymers with poly-N-p-vinylbenzyl -D-lactonamide (PVLA) were achieved by Quartz Crystal Microbalance (QCM) method and the attachment were stable on PLLA and Pst while they were unstable in PE and PET conversely. Later two kinds of matrixes were coated on electrospun network to control the cellular adhension, and proliferation properties, the functions of which were compared with the ones coated by gelatin. Those specific matrixes were PVLA as a hepatocyte-specific adhensive matrix based on matrix engineering concept, and E-cad-Fc, a kind of chimeric protein made of E-cadherin extracellular domain and immunoglobulin G(IgG) Fc region by the genetic-engineering technique. EB3, a model cell for mouse ES cell was used. The fusion protein coated 3D scaffold culture system showed similar scattering results with those of 2D system. In future further investigation on the proliferation and differentiation behaviors on the cells as well as the cellular adhesive property in long period will be reported. And the experiment will be took place both in the fields of ES cells and iPs cells. Finally the materials will be replaced as polyethylene (PE), polyethylene terephthalate (PET) and polystyrene (Pst) to evaluate the possibility of the application in regenerative medicine, such as the bioreactor for 3D culture.
ECM PVLA 3D scaffold
Meng, Qingyuan Hexig, Bayar Akaike, Toshihiro
Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology. Tokyo Institute of Technology, Japan
国际会议
International Symposium on Crystal Engineering and Drug Delivery System 2009(2009晶体工程与药物传送系统国际会议)
天津
英文
397
2009-09-05（万方平台首次上网日期，不代表论文的发表时间）