Novel Protein Crystal Formulation for Sustained Release of Interferon
1 Objects The chemical instability and rapid clearance from systemic circulation bring huge obstacles for protein drugs to be fully used as therapeutic agents. The aim of this work is to solve the significant challenge of extending the half-life of therapeutic proteins by crystallization strategy without new molecular entities generation. 2 Methods Interferon-α-2b (IFN), a model protein drug in this case, was crystallized by hanging drop vapor diffusion method for screen of initial conditions and setting method for batch production, respectively. A novel chelating technique with metal ions was employed to promote crystals formation. The prepared IFN crystals were characterized by morphology, size distribution and in vitro release behavior. The remained biological activity of IFN after crystallization was evaluated by a cytopathogenic effect inhibition assay using VSV-WISH cells. Moreover, the pharmacokinetics of this IFN crystalline formulation was studied by enzyme-linked immunosorbent assay of IFN levels in rabbit serum.
Crystallization Interferon Delivery Sustained release
Shi Kai Jiang Yanbo Cui Fude
Department of Pharmaceutics, School of Pharmaceutical Science, Shenyang Pharmaceutical University, No. 103, Wenhua Road, Shenyang 110016, China
国际会议
International Symposium on Crystal Engineering and Drug Delivery System 2009(2009晶体工程与药物传送系统国际会议)
天津
英文
404-406
2009-09-05（万方平台首次上网日期，不代表论文的发表时间）