Changes in Activity of Key Enzymes related with N and S Assimilation in Response to S-availability in Rape (Brassica napus L.)
To investigate the effect of S-availability on N and s assimilations, Enzymatic activity of nitrate reductase (NR), glutamine synthetase (GS), ATP sulfurylase (ATPS) and O-acetylserine(thiol)lyase (OASTL) was measured, and also followed by their activity staining in forage rape (Brassica napus L.) fed with three different SO42 -levels (0, 0.1 and 2.0 mM) for 25 hours. NR activity decreased as Savailability decreased, especially in young and middle leaves. S-limited conditions (0 and 0.1 mM sO42-) resulted in a reduction of GS activity. The activity of ATPS tended to increase with decreasing the exogenous SO42- supplying level. The activity of OASTL also increased as S-availability decreased for all leaf tissues, while tended to decrease in root. The results indicated that N assimilation was restricted by the limited S-nutrition especially in the developing leaf tissues, but S assimilation was stimulated except for OASTL activity in the roots.
ATP sulfurylase Glutamine synthetase Nitrate reductase O-acetylserine(thiol)lyase S vailability Rape
Lu-Shen Li Sang-Hyun Park An-Na Cho Tae-Hwan Kim
Environmental-Friendly Agriculture Research Center (EFARC), BK21 Research Team for the Control of Animal Hazards using Biotechnology, College of Agriculture& Life Science, Chonnam National University Gwangju 500757, Korea
国际会议
首尔
英文
98-99
2009-08-10（万方平台首次上网日期，不代表论文的发表时间）