Isolation and Characterization of GSTs Gene Homology Sequences from Agropyron mongolicum Keng

In this study, GSTs gene homology fragment from Mongolian wheatgrass(Agropyron mongolicum Keng) was isolated using homologybased method, one partials gene sequences have been obtained by reverse transcription polymerase chain reaction (RT-PCR). sequence analysis using Software of DNAman and DNAuser etc, and showed that the cDNA sequences was 344 base pairs, encoding 62 amino acids. The partial gene had C-terminal conserved domains of substrate binding pocket (H-site) of GSTs superfamily. Homology comparison with GSTs gene amino acid sequences in other plants showed that it was 91% identical to 19E50 of wheat, 90% to pk0078 and 88% to gstf6. It was named as MwGSTs.
Agropyron mongolicum Keng GSTs Clone Homology sequence
Yan Zhao Jin-feng Yun De-Fu Liu
College of Environment, Inner Mongolia Agricultural University, Huhhot, 010018, China Inner Mongolia College of Environment, Inner Mongolia Agricultural University, Huhhot, 010018, China
国际会议
首尔
英文
134-135
2009-08-10(万方平台首次上网日期,不代表论文的发表时间)