Ezpression Analysis of Abiotic Stress Responsive Genes in Barley Leaves Using The Annealing-Control-Primer-Based Genefishing Technique
In the present study, we have used an annealingcontrol-primer (ACP)-based differentially display RTPCR method to identify salt-stress-induced differentially expressed genes (DEGs) in barley leaves. Using 120 ACPs, a total of 11 up-regulated genes were identified and sequenced. Temporal expression patterns of some up-regulated DEGs in response to salt stress were further analyzed by Northern blot analysis. The possible roles of these identified genes are discussed within the context of their putative role in response to salt stress. Thus, the identification of some novel genes -such as snRK1-type protein kinase; 17 kDa, class I, small heat shock protein; and RNase S-like protein precursor genes-may offer a new avenue for better understanding the salt stress response in plants, knowledge which might be helpful for developing future strategies.
Barley GeneFishine technique Salt stress
Sang-Hoon Lee Ki Won Lee Ki-Yong Kim Hee Chung Ji Sung Seo Hyung Soo Park Young Chul Lim Gi Jun Choi
Grassland & Forages Research Center, National Institute of Animal Science, RDA, 330-801, Korea
国际会议
首尔
英文
138-139
2009-08-10（万方平台首次上网日期，不代表论文的发表时间）