Development of Antibiotics Marker-Free Tall Fescue Plant with Resistance to Herbicides
Antibiotics marker-free with herbicide-resistant tall fescue plants were produced through Agrobacteriummediated transformation system. Embryogenic calli derived calli were infected with Agrobacterium tumefaciem strain EHA105 harboring the pCAMBIA3300 vector containing the bar and the CP4-EPSPS genes. The PPT-resistant calli and plants were selected with 10 mg/L PPT, respectively. Soil-grown plants were obtained about 14-16 weeks after Agrobacteriummediated transformation. Genetic transformation of the regenerated plants growing under selection was demonstrated by PCR, and Southern blot analysis revealed that one copies of the transgene were integrated into the plant genome of each transgenic plant. Expression of the bar and CP4-EPSPS genes in transgenic plants was confirmed by Northern blot analysis and CP4-EPSPS genes in transgenic plant ELISA uses antibody protein by ELISA Assays. Transgenic plants sprayed of two herbicides with glufosinate ammonium or glyphosate remained green and healthy. We therefore report here a successful and reliable Agrobacteriunrmediated transformation of two herbicide-resistances and this method may be useful for routine transformation and has the potential to develop new varieties of tall fescue with several important genes.
Tall fescue forage crop herbicide CP4-EPSPS Agrobacterium
Ki-Won Lee Sang-Hoon Lee Kyung-Hee Kim Choong-Hoon Park Jin-Seog Kim Byung-Hyun Lee
Grassland & Forages Research Center, National Institute of Animal Science, RDA, 330801, Korea Divisi Grassland & Forages Research Center, National Institute of Animal Science, RDA, 330801, Korea Division of Applied Life Science, Gyeongsang National University, Jinju 660-701, Korea Korea Research Institute of Chemical Technology, Daejeon 305-600, Korea
国际会议
首尔
英文
148-149
2009-08-10（万方平台首次上网日期，不代表论文的发表时间）