Effect of Antiozidants on Callus Induction and Plant Regeneration,Factors Affecting Transformation in Miscanthus sinensis
1. Introduction Miscanthus sinensis is a perennial C4-grass have tremendous potential as a forage and energy crop for cellulosic ethanol production. High biomass production, and least requirement of irrigation, fertilizer and pesticide make it attractive for large scale commercial cultivation. However, high lignin content is a major constraint for forage digestibility and ethanol conversion. In order to develop low lignin transgenic Miscanthus sinensis, establishment of efficient tissue culture and transformation system is essential. In this study we have investigated the effects of different antioxidants on callus induction and plant regeneration using mature seeds as explant. In recent years, the trend in producing transgenic grasses has shifted toward Agrobacterium-mediated transformation, because it generally results in a lower copy number of gene(s), fewer rearrangements, economical and an improved stability of gene expression. In order to optimize the conditions for Miscanthus sinensis transformation, several factors known to influence Agrobacterium-mediated DNA transfer were examined.
Agrobacteriwn tumefaciens Antiozidants Callus induction Plant regeneration Transgenic calli
C. -H. Park M. -S. Choi B. -H. Lee Y. -G. Kim K. -H. Kim I. Alam C. -H. Joen H. -J. Lee K. -W. Lee S. A. Sharmin J. -K. Yang
Division of Applied Life Sciences (BK21 Program), College of Agriculture & Life Sciences,Gyeongsang National University, Jinju 660-701, Korea
国际会议
首尔
英文
150-151
2009-08-10（万方平台首次上网日期，不代表论文的发表时间）