Mapping Leaf Proteome of Miscanthus sinensis
1. Introduction Proteomics is the most promising technique to identify the proteins that are induced, repressed, or post-transcriptionally modified during a developmental process or stress responses. Two dimensional polyacrylamide gel electrophoresis (2-DE) combined with mass spectrometry has been used in plant proteomics analysis (Xu et al. 2006). Although global gene expression analysis is useful for selecting candidates for functional studies, the presence or absence of a given transcript does not necessarily have predictive power for the presence or absence of the protein it encodes. Thus, it is equally important to determine the protein complement of a given tissue. Greater leaf area with higher photosynthetic efficiency, least requirement for fertilizer, pesticides and irrigation and longer growing season make it advantageous over most other cellulosic crop for bioethanol production. So far, no protein reference map has been reported for Miscanthus leaves. As a first step to study stress and developmental physiology of miscanthus, we describe here the extraction and separation of leaf proteins on 2D-PAGE gels and identification of proteins using MALDI-TOFMS.
Bioenergy Cellulosic ethanol Miscanthus sinensis Proteomics
S.A. Sharmin I. Alam K.-H. Kim C.-H. Park C.-H. Joen Y.-G. Kim H.-J. Lee B.-H. Lee
Department of Animal Bioscience, Division of Applied Life Science (BK21 program), Gyeongsang National University, Jinju 660701, Korea
国际会议
首尔
英文
162-163
2009-08-10（万方平台首次上网日期，不代表论文的发表时间）