Relation between Cellulases Activity and Strains in a Lignocellulose-degradation Bacterial Community
In order to find out the dynamic changes of four kinds of cellulases and hemi cellulase in fermentation progress, the standard cellulase activity assays published by the International Union of Pure and Applied Chemistry (IUPAC) was used. The results showed that enzyme activities were all one signal peak curve, endoglucanases, exoglucanases, a -glucosidases and the total cellulase activity reached the maximum on the day 8, the value were 4.48, 15.83, 25.78 and 7.51 U/mL , respectively. While compared with cellulase, the maximum of hemi cellulase appeared earlier , it reached to the maximum 280.9 U/mL on the day 5. For find out the relation between enzyme activity and strains in bacterial community, a technology of polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was used to profile the stru ctured dynamic changes of microbial communities in fermentation process. The result showed that the strain of Bacteria and Paenibacillus could promote excrete xylanase. And the strain of Clostridium could excrete cellulase and xylanase, simultaneity.
cellulose-degradation bacterial community cellulase hemicellulase PCR-DGGE microbial component diversity
LIU Changli WANG Xiaofen LV Yucai NIU Junling SHEN Hailong CUI Zongjun
College of Life Science/School of Forestry,Northeast Forestry University,Harbin 150040,Heilongjiang, College of Agronomy and Biotechnology ;Center of Biomass Engineering,China Agricultural University,B Zhengzhou Insitute of Aeronautical Industry Management,Zhengzhou 450015,Henan,China College of Life Science/School of Forestry,Northeast Forestry University,Harbin 150040,Heilongjiang,
国际会议
2009 International Symposium on Environmental Science and Technology(2009环境科学与技术国际会议)
上海
英文
247-253
2009-06-02（万方平台首次上网日期，不代表论文的发表时间）