Investigation on the Role of Calcium in Aluminum Tozicity in Cultured Tobacco (Al sensitive) and Tea (Al tolerant) Cells
Aluminum toxicity is one of the factors that seriously limits plant growth in acid soils. It is suggested that aluminum accumulate primarily and predominantly in the root apoplast where the pectin matrix with its negative charges is a major binding site. At the cellular level plasma membrane is one of most important targets for aluminum where interferes with lipids via increasing production of reactive oxygen species. This study was undertaken to elucidate possible interaction(s) between aluminum and calcium in suspension-cultured tea (as Al tolerant) and tobacco (as Al sensitive) cells. The cells were treated either with 60 μM Al, or 127 μM Ca, or a combination of both, for 24 h. Viability of cells and their capacity for absorption of Al and Ca, flavonoid content, and the rate of proxidation of membrane lipids were measured. Degree of methyl esterification of cell wall pectin was quantified applying monoclonal antibodies JIM7 and JIM5 as well as by chemical analysis. According to the results, Al induced an increase in the amount of methyl esterified pectin in tea cells whereas tobacco cells de-esterified pectin increased by Al treatment. This result showed that pectin plays an important role in Al toxicity and Al tolerance. Aluminum treatment also increased the level of peroxidation of lipids of tobacco cells. Content of flavonoids of tea cells increased by the treatment with either Al or Ca declined in tobacco cells
Aluminum Calcium Flavonoids JIM5, JIM 7 Lipid perozidation Methyesterified Pectin
Faezeh GHANATI Taraneh BANAEE Farnoosh NEMATI
Department of Plant Biology,Faculty of Science,Tarbiat Modares University (TMU) Tehran,Iran
国际会议
2009 International Symposium on Environmental Science and Technology(2009环境科学与技术国际会议)
上海
英文
326-330
2009-06-02（万方平台首次上网日期，不代表论文的发表时间）