Quantitative Determination of Amylase Enzymes from Egyptian Soil
Twenty seven bacterial isolates from Egyptian soil with addition to a reference Bacillus subtlus strain were used to compare the production of extracellur amylases. Two strains were found to be hyperproductive to amylases. One is Bacillus specie and the second is Cocci specie. Enzymes obtained from the three strains were precipitated with solid ammonium sulphate (65%), followed by dialysis. The bacterial extracts were then applied on column chromatography (sephadex, G100). Active fractions were collected for each strain and characterized biochemically. The optimum pH is 4, and the optimum temperature is 55 ℃. It was found that, EDTA, SLS, and heavy metals quench the activity of the enzyme samples with different percent. The protein content of the enzyme samples were determined using spectrophotometeric analysis. Two simple tests, iodine test for starch and Bradford using Bovine serum albumin as a standard absorbance at 595 nm test for protein assay are used for determination of amylases and protein in microbial assay.
amylases enzyme purification biochemical characterization Egyptian soil spectrophotometric determination
Nashwa M.H.RIZK Salah M.El-KOUSY Ayman M.M.SAWAN
Environmental Biotechnology Department,Genetic Engineering and Biotechnology Research Institute (GEB Chemistry Department,Faculty of Sciences,Minufiya University,Egypt
国际会议
2009 International Symposium on Environmental Science and Technology(2009环境科学与技术国际会议)
上海
英文
354-361
2009-06-02（万方平台首次上网日期，不代表论文的发表时间）