Copper Accumulation and Eco-physiological and Biochemical Responses of Androgenic Carrot Cultures to Copper Stress
Carrot easily accumulates toxins, so it is very important to use in vitro cultures in selecting and eliminating the cultivars which accumulate excessive amounts of metals. Long term influence of Cu2+ on regeneration and growth of carrot genotype Kazan obtained from androgenic embryos was analyzed. Copper was added to the medium at concentrations: 0.1 μM (control), 1 μM, 10 μM and 100 μM. The cultivation at 10 μM Cu2+ concentration resulted in stimulation of growth during 4 and 9 weeks, even if TBARS level also increased. At the same time a significant increase in phenolic compounds absorbing UV light at 330 nm was observed. It seems that flavonoids rather than free proline may be involved in protective mechanisms against Cu2+ stress during short term regeneration of carrot androgenetic embryos. The highest Cu2+ concentration (100 μM) enhanced lipid peroxidation, and although after 9 weeks of cultivation the proline level significantly increased, the Cu accumulation in tissues was almost doubled in comparison with the control and growth limitation was caused. Rosettes growing at low concentrations of Cu2+ (0.1 and 1 μM) accumulated less Cu over time, which was parallel to their high organogenic ability and could suggest their acclimation to stress conditions.
carrot anther culture copper proline TBARS flavonoids
SZAFRANSKA Katarzyna KOWALSKA Urszula GORECKA Krystyna OSINSKA Katarzyna JANAS Krystyna
Department of Ecophysiology and Plant Development,University of Lodz,Banacha 12/16,90-237 Lodz,Polan Research Institute of Vegetable Crops,Laboratory of Biotechnology,Skierniewice Konstytucji 3 Maja,Po
国际会议
2009 International Symposium on Environmental Science and Technology(2009环境科学与技术国际会议)
上海
英文
377-382
2009-06-02（万方平台首次上网日期，不代表论文的发表时间）