Quantifying Metallothionein Transcript by Real Time PCR during Single and Combined to Cadmium and Copper in Sinopotamon yangtsekiense
A method of real time fluorescent quantitative PCR (qPCR) has been applied to investigate gene expression changes after heavy metals contaminated. In this article, the qPCR method was optimized and designed to study the relative expression of Sinopotamon yangtsekiense metallothionein gene (SYMT) treated with different concentrations of heavy metals cadmium (Cd) and copper (Cu). The samples were exposed to four concentrations of Cd (0.005, 0.05, 0.5, 5 mg/L) and Cu (0.1 mg/L) for 24, 48, 96 h. The results suggested that SYMT gene was more sensitive to Cd compared to Cu, and in experimental concentration scale (≤5 mg/L Cd)，raising Cd concentrations increased the relative quantity (RQ) levels of SYMT expression. Furthermore, it showed the higher induction rate in gill than in heart during 24, 48, 96 h. The effect of low concentration Cu itself on RQ levels of SYMT was slightly elevatory, but the interactive effect of combined Cd and Cu was significantly different from single Cd at p<0.05. Therefore, Cu could enhance the effect of Cd on SYMT, which showed cooperative effect. The RQ levels of 48 h treatment were similar to those of 96 h treatment exposed to the same concentration. After Cd exposure, SYMT levels continuously increased, and showed a clear dose response and significant elevation in experiment concentration scale, as implied RQ levels of SYMT were suitable as a biomarker for evaluating Cd pollution.
Sinopotamon yangtsekiense metallothionein gene biomarker real time qPCR cadmium and copper
GAO Aibao WANG Lan YUAN Hui LEI Wenwen
School of Life Science and Technology,Shanxi University,Taiyuan 030006,Shanxi,China Jinzhong univers School of Life Science and Technology,Shanxi University,Taiyuan 030006,Shanxi,China
国际会议
2009 International Symposium on Environmental Science and Technology(2009环境科学与技术国际会议)
上海
英文
456-460
2009-06-02（万方平台首次上网日期，不代表论文的发表时间）