A Nafion/BSA Based Conductometric Algal Whole Cell Biosensor for Heavy Metal Monitoring
This work describes the design of a conductometric biosensor intended to monitor aquatic environments. This biosensor is based on the measurement of the Alkaline Phosphatase Activity (APA) of the microalgae Chlorella vulgaris. This activity is inhibited in the presence of heavy metals and phosphate. The essential purpose of this article is to obtain a tool for detection of heavy metals through inhibition of APA. It is therefore necessary to eliminate the artifact linked to the APA inhibition in the presence of phosphates (always present in the aquatic media). In earlier works we developed optical and conductometric sensors based on measuring activity of enzymes located on the external membrane of algae. The main problem encountered is the interference of phosphates in the measurment of APA inhibition. To solve this problem, a Nafion membrane is used. Negatively charged, the membrane pushes the phosphate ions charges also negatively. This sensor is not specific for a single metal, but it provides a global response to the presence of heavy metals. This biosensor can be considered as an early warning system, and was used to measure concentrations of heavy metals in the order of ppb in real samples as urban water.
conductometric biosensor Chlorella vulgaris nafion BSA alkaline phosphatase activity cadmium
GUEDRI Houssemeddine DURRIEU Claude
Université de Lyon,Laboratoire des Sciences de l’Environnement,Ecole Nationale des Travaux Publics de l’Etat,rue Maurice Audin,69518 Vaulx-en-Velin Cedex,France
国际会议
2009 International Symposium on Environmental Science and Technology(2009环境科学与技术国际会议)
上海
英文
623-628
2009-06-02（万方平台首次上网日期，不代表论文的发表时间）