Aerobic Degradation of Chlorobenzene by Acinetobacter calcoaceticus Isolated from Polluted Water Body

摘要：

A chlorobenzene(CB)-degrading bacterium was isolated by enrichment technique from chlorobenzene-contaminated water body. It was classified and identified as Acinetobacter calcoaceticus CB001 by phylogenetic analysis based on 16S rDNA sequence, in addition to biochemical and physiological characteristics. Resting cells were able to degrade CB from culture fluids with the concomitant release of chloride ion. The ranges of pH values and temperatures for good degradation of CB by strain CB001 cells were from 7.0 to 8.0 and 25 to 30℃, respectively. First order kinetics could be fitted to the biodegradation of CB after lag phase at initial concentrations of 20, 50, 80, and 120 mg·L-1. 80 mg·L-1 is the most suitable concentration for strain CB001 to degrade CB with the first order rate constant of 0.0376 h-1. Strain CB001 also showed capacity to degrade 1, 2-dichlorobenznene, 1, 3-dichlorobenznene, 1, 4-dichlorobenznene at solely initial concentration of 50 mg·L-1, but failed to degrade hexachlorobenzene. Results from this study could be helpful in the optimization of its use in bioremediation of CB.

关键词： biodegradation chlorobenzene 16S rDNA phylogenetic analysis

作者: LI Mingtang CUI Juntao HAO Linlin SHENG lianxi XU Jingbo1

作者单位: Department of Environmental Science and Engineering,Northeast Normal University,Changchun 130024,Jil College of Resources and Environment,Jilin Agricultural University,Changchun 130118,Jilin,China College of Animal Husbandry and Veterinary Medicine,Jilin University,Changchun 130062,Jilin,China Department of Environmental Science and Engineering,Northeast Normal University,Changchun 130024,Jil

会议类型: 国际会议

会议名称: 2009 International Symposium on Environmental Science and Technology(2009环境科学与技术国际会议)

会议地点: 上海

会议语种:英文

页码: 1343-1348

在线出版日期: 2009-06-02（万方平台首次上网日期，不代表论文的发表时间）