Production of a Bioflocculant by Pseudomonas fluorescens C-2 and Its Application in Wastewater Treatment

摘要：

Pseudomonas fluorescensC-2 was found to produce a novel bioflocculant PS-2 with high flocculating activity for Kaolin suspension and six wastewaters .Results showed that the carbon and nitrogen sources favorable for the production of the PS-2 were glucose and soybean flour, and an optimal condition of 30 ℃, 5% of inoculum size (v/v), initial pH 8.0 and shaking speed of 150 rpm. The highest flocculating efficiency achieved for Kaolin suspension was 95.8%, after 72 h cultivation. The bioflocculant PS-2 was mainly composed of sugar (86.7%) and nucleic acid (7.8%), and an average molecular weight of 2.38×106. Fourier transform infrared (FTIR) spectra showed hydroxyl, C-H, carbonyl, C-N-H groups were present in the PS-2 molecule. The PS-2 was effective for removal of COD, chromaticity and turbidity of six wastewaters, in particular domestic sewage and riverwater with a turbidity removal efficiency of 95.80% and 95.26%, respectively. The flocculation process indicated negative groups (hydroxyl) in PS-2 molecules made flocculant molecules extend fully and played a role on bridging, it was concluded that the flocculation process was based on the bridging mechanism, the combination of organic particles with PS-2 by hydrogen bond at the active position of bioflocculant.

关键词： pseudomonas fluorescensC-2 flocculation performance bioflocculant PS-2 UV and FTIR wastewater treatment

作者: MAO Yanli LUO Shitian ZHANG Kui XIAO Xiaocun YAN Yongsheng

作者单位: Municipal Engineering Department,Henan University of Urban Construction,Pingdingshan 467001,Henan,Ch Construction Engineering Quality Supervise Station of Pingdingshan Construction Commmittee,Pingdings Municipal Engineering Department,Henan University of Urban Construction,Pingdingshan 467001,Henan,Ch School of Chemistry and Chemical Engineering,Jiangsu University,Zhenjiang 212013,Jiangsu,China

会议类型: 国际会议

会议名称: 2009 International Symposium on Environmental Science and Technology(2009环境科学与技术国际会议)

会议地点: 上海

会议语种:英文

页码: 1354-1359

在线出版日期: 2009-06-02（万方平台首次上网日期，不代表论文的发表时间）