Immobilization of Laccase on Modified Chitosan and Its Use for 2, 4-Dichlorophenol Degradation

摘要：

The laccase was immobilized on crosslinking chitosan with glutaraldehyde and its properties for 2, 4-dichlorophenol degradation were evaluated. The activity of the immobilized laccase depending on the immobilizing procedure was discussed at length. The results showed that the activity of immobilized laccase prepared by crosslinking of chitosan and then adsorption was obviously higher than that by adsorption and subsequent crosslinking. For the crosslinking of chitosan, the optimum concentration of glutaraldehyde and crosslinking time was 5% and 12 h, respectively. For the adsorption of laccase, the immobilized laccase exhibited the maximum enzyme activity when laccase was adsorbed for 8 h at pH of 5.0. The optimum dosage of laccase was 90 mg/g carrier. The immobilized laccase showed obvious catalytic capacity for 2, 4-dichlorophenol degradation. When the amount of immobilized laccase was 8 g/L, removal efficiency of 2, 4-dichlorophenol in water (solution volume: 50 mL) reached 54.8% after 16 hours.

关键词： immobilization laccase chitosan 2, 4-dichlorophenol

作者: ZHANG Haisi WANG Ying PEI Yuansheng GUO Xuejun FENG Chenghong

作者单位: State Key Laboratory of Water Environment Simulation,Beijing Normal University,Beijing 100875,China State Key Laboratory of Water Environment Simulation,Beijing Normal University,Beijing 100875,China State Key Laboratory of Water Environment Simulation,Beijing Normal University,Beijing 100875,China

会议类型: 国际会议

会议名称: 2009 International Symposium on Environmental Science and Technology(2009环境科学与技术国际会议)

会议地点: 上海

会议语种:英文

页码: 1765-1769

在线出版日期: 2009-06-02（万方平台首次上网日期，不代表论文的发表时间）