Evaluation of DNA Eztraction Methods as Well as the Specificity of Two Primer Sets in the Detection of VBNC form of Escherichia coli in the Recreational Waters of the Southern Caspian Sea
1 Introduction Intestinal bacteria, especially Escherichia coli, are used as indicators of fecal contamination. Hence many researchers have undertaken the study of their survival in natural aquatic systems. In response to adverse environmental conditions E. Coli cells maintain their integrity and some metabolic activity, but they lose the ability to grow in culture media. In other words, they enter a viable but non-culturable (VBNC) state. As a consequence, alternative methods were developed during the last 10 years to detect and enumerate E. Coli in waters. Those are direct enzymatic methods, immunological methods, polymerase chain reaction (PCR) and fluorescent in situ hybridization (FISH). This study was conducted to evaluate E. Coli as an index of microbial pollution in recreational waters of the Caspian Sea containing about 1% salt. This bacterium is able to produce VBNC form under different stress conditions as well as salinity.
recreational waters PCR E.coli specificity of primer.
LANGAROODI G H Zohre SOUDI Mohammad Reza GHARAVI Sara SEPEHR Shayesteh
Microbiology Group,Department of Biology,Alzahra University,Tehran,Iran
国际会议
2009 International Symposium on Environmental Science and Technology(2009环境科学与技术国际会议)
上海
英文
2498-2499
2009-06-02（万方平台首次上网日期，不代表论文的发表时间）