MESOPHASE TRANSFORMATIONS IN A DNA COMPLEX WITH CATIONIC POLYHEDRAL OLIGOMERIC SILSESQUIOXANE LIPID
Introduction To improve and eventually realize effective nonviral gene therapy, understanding the structure-function relationship of DNA-cationic lipid complexes remains an important research task.1 Equilibrium morphologies of lipoplexes, typically lamellar and inverted hexagonal phases, are a result of the free energy balance among surface charge density, spontaneous curvature of the lipids, and elastic properties of the lipid bilayers. When the lipid spontaneous curvature (c0) is low (zero) and the bending modulus (k) is high, a lamellar phase with parallel DNA chains intercalated between positively charged lipid bilayers (a mixture of cationic and neutral helper lipids) is preferred.2-4 When the c0 is high (negative and |c0| ≈1/Rdna where RDNA is the radius of DNA around 1.0 nm) and the k is low, an inverted hexagonal phase is favored.l0 It has been reported that mesophase morphology of lipoplexes is one of the important factors for high transfection efficiency.5、6 Controlling transformations between lamellar and inverted hexagonal phases has thus received much attention.
Li Cui Daoyong Chen Lei Zhu
Polymer Program,Institute of Materials Science and Department of Chemical,Materials and Biomolecular Department of Macromolecular Science and Key Laboratory of Molecular Engineering of Polymers,Fudan U
国际会议
International Symposium on Polymer Physics(2008年国际高分子物理学术会议PP2008)
厦门
英文
236-237
2008-06-08（万方平台首次上网日期，不代表论文的发表时间）