Electromagnetic Pulses Effects on Insulins Bioactivity and Mechanism Study
Purpose: To investigate the effects of electromagnetic pulse (EMP) exposure on insulins bioactivity and their preliminary mechanism.Materials and methods: A EMP generator was used to expose the insulin solution.Concurrent sham-exposed insulin solution was used as control.The effects of EMP-exposed insulin on fasting blood glucose of type I diabetes model mice, the effects of EMP on binding affinity between insulin and its receptor, and the effects of EMP on insulins fluorescence intensity were detected respectively.Results: ①After EM P exposure,compared with sham-exposed insulin, the insulins bioactivity of decreasing fasting blood glucose in type I diabetes model mice reduced significantly (P=0.023) ②Compared with sham-exposed insulin group, the percentage fluorescein isothiocyannate labeling of HL-7702 cells was significantly reduced in EMP-exposed insulin group (22.7％ to 13.8％,respectively)③Compared with sham-exposed insulin, the fluorescence intensity was significantly reduced in EMP-exposed insulin (P<0.001).Conclusions: EMP exposure could significantly decrease the bioactivity of insulin by reducing the blood glucose levels in type I diabetic mice.This could be due to decreased binding affinity between insulin and its receptor.The mechanism could involve an alteration of insulins conformation caused by EMP exposure.
electromagnetic pulse (EMP) insulin binding affinity conformation
YONG-BIN CHEN JUAN TAN XIA MIAO JING LI GUO-ZHEN GUO
Department of Radiation Medicine,Fourth Military Medical University,Xian,China
国际会议
Asia-Pacific Conference on Environmental Electromagnetics CEEM2009(第五届亚太环境电磁学学术会议)
西安
英文
217-220
2009-09-16（万方平台首次上网日期，不代表论文的发表时间）