Scavenging effects of Echinacea. purpurea eztract and active ingredient against perozynitrite
The present study was to investigate the protective effect of Echinacea. purpurea (E. purpurea) extract, a natural scavenger of peroxynitrite (ONOO-), on the biomolecules injury induced by ONOO-in vitro. The results showed that E. purpurea extract could prevent the ONOO-mediated damage in tyrosine nitration, low density lipoprotein (LDL) oxidation and DNA strand breaks. The ONOO-scavenging ability of E. purpurea in the process of 3-nitrotyrosine (3-NT) formation exhibited dose-dependent manner. The results of LDL oxidation assay indicated that the extract could inhibit LDL oxidation correlates with lipid hydroperoxide concentration. In addition, E. purpurea was able to inhibit ONOO-induced DNA damage by 50％ at a concentration of 150μg/ml. For further study on the relationship of scavenging activity between the complex and the monomer, we isolated a unique component from E. purpurea, cichoric acid as an active constituent, which has stronger inhibition effects against ONOO-induced tyrosine nitration and DNA single strand breaks.
perozynitrite Echinacea. purpurea DNA damage 3-nitrotyrosine lipid ozidation cichoric acid
Yun-Jing Luo Jing-lin Pan Yan-shu Pan Ru-gang Zhong
College of Life Science & Bioengineering,Beijing University of Technology,Beijing 100022,China School of Basic Medical Science,Beijing University of Chinese Medicine,Beijing 100029,China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）