Net Analyte Signal Calculation in Near-Infrared Spectra Calibration
In this paper, net analyt signal (NAS) calculation was used for Near-infrared (NIR) spectra calibration and prediction glucose concentration in urine sample. Near-infrared spectra in the 10000～4000 cm-1 region were measured for human urine glucose with various concentrations at 37°C. The most informative spectral ranges of 4200～4800 cm-1 were selected by principal component analysis (PCA) for glucose in the mixtures. For glucose, the correlation coefficient (R) of 0.9988 and the root mean square error of prediction (RMSEP) of 20.03 mg/dl were obtained by NAS and partial least squares (PLS). The NAS is the part of the spectrum that is directly related to the analyte of interest, and it can be used as a rapid and reliable tool for quantitative analysis of glucose in biological sample.
Near-infrared spectra Glucose Net analyte signal Partial least-squares Urine
Weiling Liu Libing Liu Qilian Yu
School of mechnical Hebei University of Technology Tianjin,CHINA College of Precision Instruments & Opto-electronics Engineering Tianjin University Tianjin,CHINA
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）