Kinetic Analysis of Aminoethylisothiourea on Diphenolase of Mushroom Tyrosinase

摘要：

This study demonstrates that Aminoethylisothiourea (AET), a potent inhibitor of inducible nitric oxide synthase (NOS), is an irreversible competitive inhibitor of the diphenolase activity of mushroom tyrosinase when L-DOPA was assayed spectrophotometrically in vitro. The inhibition of diphenolase activity of tyrosinase by AET showed positive kinetic cooperativity which arose from the protection of both substrate and o-quinone against inhibition by AET.

关键词： aminoethylisothiourea diphenolase tyrosinase irreversible inhibition positive cooperativity

作者: Shu-Bai Li Hai-Tao Zhang Yong Xue Hua-Li Nie Li-Min Zhu Xiao-Fei Zhang Christopher Branford-Whiter

作者单位: College of Chemistry,Chemical Engineering and Biotechnology Donghua University Shanghai,China Institute for Health Research and Policy London Metropolitan University London,UK

会议类型: 国际会议

会议名称: The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)

会议地点: 北京

会议语种:英文

页码: 1-4

在线出版日期: 2009-06-11（万方平台首次上网日期，不代表论文的发表时间）