Analysis of Seven Polyacrylamide(PAM)-Degrading bacterias using PCR-DGGE Method

摘要：

Seven polyacrylamide(PAM)-degrading bacteria were isolated from waste water treated pool of Henan(P.R.China) oil field, six of which were isolated from the sample of sludge, one from the sample of waste water. Sequences and phylogenetic analysis of 16S rDNA clones of the seven bacteria showed that four were affiliated with Actinobacteria, two with Alphaproteobacteria, one with Bacillus. PCR-DGGE profile was used to analyze the environmental dominant status of the seven bacteria. DGGE bands of five bacteria can be found in the lane of DNA from original water or sludge samples. The result provided valuable information about PAM waste treatment of He Nan Henan oil field.

关键词： Oil field Polyacrylamide (PAM)-degrading Phylogenetic Denature gradient gel electrophoresis(DGGE)

作者: Yuehui She Fan Zhang Zhengliang Wang Liping Zhao Longjiang Yu

作者单位: School of Chemical and Environmental Engineering,Yangtze University,Jingzhou 434023,China School of School of Chemical and Environmental Engineering,Yangtze University,Jingzhou 434023,China College of Life Science and Biotechnology,Shanghai Jiaotong University,Shanghai 200240,China School of Life Science and Technology,Huazhong University of Science and Technology,Wuhan430070,Chin

会议类型: 国际会议

会议名称: The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)

会议地点: 北京

会议语种:英文

页码: 1-4

在线出版日期: 2009-06-11（万方平台首次上网日期，不代表论文的发表时间）