Phylogenetic analysis of four Vibrio strains of pathogenic bacteria based on hemolysin genes and 16S rRNA genes
In order to understand the phylogenetic relationships among Vibrio species more rationally, the hemolysin gene partial sequences of 4 Vibrio species (Vibrio alginolyticus, Vibrio harveyi, Vibrio parahaemolyticus, Vibrio minius) were amplifyed and sequenced. We constructed two separate phylogenetic trees by using 16S rRNA and partial hemolysin gene sequences, respectively.The average similarities of hemolysin gene of Vibrio species was 37.2％. Meanwhile, the average similarity of 16S rRNA gene of Vibrio species was 91.4％. The variabilities of hemolysin gene in Vibrio is greater than that of 16S rRNA gene. By comparing the phylogenetic trees, the topology of hemolysin gene tree is almost consistent with that of 16S rRNA gene tree. Consequently, the hemolysin gene possesses the superiority in phylogenetic taxonomy of the genus Vibrio. Taxonomic classification of Vibrios with hemolysin gene instead of 16S rRNA gene was proved a better approach.
Vibrio hemolysin gene 16S rRNA gene Phylogenetic analysis
Xiuli Wang Ye Yuan Hongyue Wang Qunlan Zhou Jian Zhu Yaqing Chang Yudong Cui Xuemei Qiu
Key Laboratory of Mariculture and Biotechnology of Agriculture Ministry,Dalian Fisheries University, Freshwater Fisheries Research Center,Chinese Academy of Fishery Science,Wuxi 214081,China College of Life Science and Biotechnology,Heilongjiang August First Land Reclamation University,Daqi
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）