Analysis of SSR Marker of Wheat Mutants of Genetic Transformation Mediated by Ion Beam
13 lines including 9 wheat mutants of genetic transformation by ion beam and 4 acceptor control varieties were analyzed by SSR marker.A special band of dwarf mutant line 1042 was obtained, sequenced and analyzed by blast software.The genetic distance was analyzed with SSR marker materials by SAS software.The result showed that 9 wheat mutants were clustered together with their own acceptor control varieties respectively.These suggested that the variance of mutants of genetic transformation mediated by ion beam was smaller than genetic variance among different varieties.The result aligned by blast software showed that the special sequence of dwarf mutant line 1042 was a part of the Rht-D1a gene.These suggested that the reason of declining the height of 1042 mutant line was Rht-D1a mutant.
Wheat Ion beam Genetic transformation RAPD SSR Sequential analysis
Tie-gu Wang Xue-bin Li Ming-wang Shi Wei-dong Wang Shi-lin Chen Xing-qi Ou Ming-jiu Liu
Henan Institute of Science and Technology Xinxiang,453003,P.R.China Henan Provincial Key Laboratory of Ion Beam Bioengineering,Zhengzhou University Zhengzhou 450052,P.R
国际会议
北京
英文
1-3
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）