Identification and sequence analysis of four SRNase genes in plumcot (Prunus simonii Carr.)
Plumcot (Prunus simonii Carr.) is an interspecific hybrid between plum (P.salicina) and apricot (P.armeniaca) that plays an important role in non-timber forestry industry and making medicine.It exhibited S-RNase-based gametophytic selfincompatibility.In this study, four S-RNase genes were identified and characterized.All of them displayed typical structural features of Prunus S-RNases, i.e.one signal peptide, five conserved regions, one Rosaceae-specific HV region and two introns.At the amino acid level, P.simonii S1 shared an extremely high similarity (99.5％) with P.salicina Sh-RNase, indicating that they likely represented the same S-RNase.In comparison with other Prunus S-RNases, P.simonii S1 showed a 65.6％ to 80.4％ similarity.Likewise, P.simonii S2 and P.simonii S4 were likely identical to P.armeniaca S2-RNase and P.salicina Sb-RNase, respectively, and showed 66.5％ to 78.7％ similarity to other Prunus S-RNases.In the case of P.simonii S3, it shared 66.5％ to 89.6％ similarity to other Prunus S-RNases.Similarity analysis well mirrors that plumcot originated from the cross of plum and apricot.Phylogenetic analysis indicated that the SRNase divergence predated speciation in Prunus.Sequence information provided in the four plumcot S-RNases will be useful for function analysis in self-incompatible response.
plumcot interspecific hybrid self-incompatibility SRNase bioinformatics
DUAN Jing-hua LI Fang-dong DU Hong-yan
Non-timber Forestry Research and Development Center,Chinese Academy of Forestry,Zhengzhou city,450003,Peoples Republic of China
国际会议
北京
英文
1-3
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）