Isolation and Functional Analysis of LOX gene from seeds of Wild soybean

摘要：

Lipoxygenases are non-heme iron-containing dioxygenases that are widely distributed in plants.Construction of cDNA Library and cloning of wild soybean seed lipoxygenase gene LOX, the structural characteristics of the gene expression and enzyme characteristics were analyzed.Bioinformatics analysis showed that the N-terminal contains about 60 additional amino acids of LOX, LOX may have activity.Lox and soybean gene with the highest cDNA sequences (about 79％), Phaseolus vulgaris, Arachis hypogaea, Glycine max, Solanum tuberosum, Solanum tuberosum, Solanum tuberosum, Prunus dulcis Miller., Prunus armeniaca, Prunus armeniaca, Pyrus pyrifolia, Persea americana, Arabidopsis thaliana, Adelostemma gracillimum the cDNA sequences were 76,76,79,77,71,70,70,71,69,70,69,67,67 and 70％，respectively.

关键词： wild soybean lipozygenase LOX gene family EST Cloning

作者: Ming-wang SHI Qing-lian WANG Tian-FU Deng Xing-qi OU Xue-bin LI Qiu-yun LIU Xue-yong LI Jian-feng LANG

作者单位: Henan Institute of Science and Technology,Xinxiang,453003,Peoples Republic of China

会议类型: 国际会议

会议名称: The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)

会议地点: 北京

会议语种:英文

页码: 1-3

在线出版日期: 2009-06-11（万方平台首次上网日期，不代表论文的发表时间）